ABSTRACT
Background: Colorectal cancer ranks as the 7th most common cancer among Iraqis. This task intended to check out the tissue level of Interleukin-6 (IL-6) in colorectal tumors using IL-6 specific antibodies and Immunohistochemistry (IHC). Methods: IHC staining was performed using Interleukin-6 (IL6) specific antibodies. Tissue samples included 50 cases of colorectal cancer, 8 cases of colon adenomas in addition to 20 samples of normal colon tissues as a control. Results: Total 39 out of 50 colorectal cancer cases (78%) demonstrated positive expression of IL-6 specific antibody. While only 2 out of 8 adenomas (25%) exhibited positive outcome when stained with this marker, comparing to 20 normal colon tissue samples which revealed a negative expression of IL-6. Results disclosed significant association between IL-6 expression and clinicopathological traits including age, sex as well as tumor stage and grade. Conclusion: Our findings revealed that IL-6 might be a valuable indicator for predicting the prognosis for patients with colorectal cancer and might act as a future therapeutic approach in colorectal cancer.
ABSTRACT
The intent of this task was to examine the toxic effect of different dosages of Duprost (dutasteride) on the spleen of mice. Total 24 albino mice were purchased and fractioned into 3 parts: control (3 mice), acute group (12 mice) and chronic group (9 mice). The acute group was subdivided to 4 groups, each of which contained 3 mice. Every group was given a lonely oral dose of the following doses: subgroup 1 dosed with 0.25 ml (0.5 mg/kg) of Duprost (dutasteride), subgroup 2 dosed with 0.15 ml (0.12 mg/kg) of Duprost (dutasteride), subgroup 3 dosed with 0.1 ml (0.08 mg/kg) of Duprost (dutasteride) and subgroup 4 dosed with 0.05 ml (0.04 mg/kg) of Duprost (dutasteride) for 24 hours. Whereas the chronic group was subdivided into 3 subgroups and each set was given a daily dose of 0.15 ml, 0.1 ml, and 0.05 ml of Duprost (dutasteride) respectively for 42 days. After the mentioned periods, the mice of all groups were sacrificed and the spleen of each animal was removed, processed, sectioned and stained for histological analysis. In the acute group, all mice that were dosed with 0.25 ml dose passed after 15 minutes of dosing. The histological analysis of spleen in residual mice of acute subgroups showed marked congestion and edema. In comparison with chronic subgroups in which depletion of white pulp had been noticed in addition to the absence of follicles and decreased the size of the periarterial lymphatic sheaths (PALS), irregular bands of fibrosis and early collagen deposition causing distortion of the splenic contour is observed. Moreover, there was an expansion of red pulp with increased cellularity and size of the red pulp. And the separation of splenic cords by irregularly shaped wide sinusoids.